TB-500 comes in 5 mg vials. You reconstitute it to nail doses like 2 mg per injection. Research shows it hits peak tissue levels in 24 hours and hangs around for days.
Reconstitution for TB-500
Take a 5 mg vial. Stick to bacteriostatic water. Regular sterile water won't cut it without that benzyl alcohol to fight bacteria.
For 2.5 mg/ml, pull 2 ml into an insulin syringe. Angle the needle into the vial so droplets slide down the side. Roll it gently between your fingers for 5 minutes till it's clear. Now 0.8 ml gives you 2 mg.
Prefer it stronger? 1 ml water makes 5 mg/ml. 0.4 ml = 2 mg. Hit up our TB-500 calculator for exact volumes.
Keep reconstituted vials in the fridge at 2 to 8°C. Good for 4 weeks. Freeze the dry powder at -20°C forever. Thaw it once only—freezing and thawing over and over wrecks it.
Wipe vial tops with alcohol first. Grab 0.3 ml or 0.5 ml insulin syringes for subQ in the belly.
TB-500 Half-Life and Injection Timing
Half-life is 48 to 72 hours from animal studies. Inject every 3 to 4 days. No need to pin daily.
After 2 mg, tissues max out at 12 to 24 hours. By day 3, levels halve. So Monday and Thursday works great.
Twice a week fits the half-life. Daily shots? Overkill.
Standard TB-500 Loading Protocol
Go hard: 7.5 mg total weekly for 4 weeks. Split 3.75 mg Monday and Thursday.
From 2.5 mg/ml:
- 3.75 mg = 1.5 ml. 3 ml syringe.
Lighter: 4 to 5 mg/week (2 to 2.5 mg twice).
Weeks 1 to 4:
- Monday: 2.5 mg
- Thursday: 2.5 mg
That's 20 mg total. Ease off if you feel draggy.
Start every cycle this way. It floods those actin sites quick.
TB-500 Maintenance Dosing
Post-load, 2.5 to 4 mg weekly for 8 weeks. Once a week or split it.
Weeks 5 to 12: 2 mg every 4 days.
Repair keeps going without excess. Off 4 weeks, repeat if you want.
2 mg weekly holds tendon benefits long-term.
Full TB-500 Research Cycle Example
6-week run, 36 mg total:
Weeks 1-2 (Aggressive Load):
| Day | Dose | Volume (2.5 mg/ml vial) |
|---|---|---|
| Mon | 3 mg | 1.2 ml |
| Thu | 3 mg | 1.2 ml |
Weeks 3-4 (Load Taper):
| Day | Dose | Volume |
|---|---|---|
| Mon | 2.5 mg | 1 ml |
| Thu | 2.5 mg | 1 ml |
Weeks 5-6 (Maintenance):
| Day | Dose | Volume |
|---|---|---|
| Mon | 2 mg | 0.8 ml |
Under 80 kg? Cut 20%. Track weekly.
TB-500 Stacking Protocols
Pair with BPC-157. BPC fixes local spots, TB-500 works everywhere.
Combo:
- TB-500: 2.5 mg twice weekly
- BPC-157: 300 mcg daily subQ by the injury
BPC-157 calculator for volumes. 4 weeks load, 4 maintenance.
CJC-1295/Ipamorelin for extra recovery:
- TB-500 2 mg twice weekly
- CJC/Ipa: 100/100 mcg nightly
CJC-1295/Ipamorelin calculator.
GHK-Cu for skin: 1 mg three times weekly. GHK-Cu calculator.
Skip Semaglutide or Tirzepatide during loads. They mess with your stomach, but subQ TB skips that anyway.
Site Rotation and Administration
Switch subQ spots: left/right abdomen, upper arms, thighs. Space 1 inch apart.
29-31G insulin syringe, 45° angle. No aspirate for subQ.
Massage lightly 30 seconds after. Drink more water—TB pulls it into tissues.
Storage After Reconstitution
Fridge 2 to 8°C: 28 days max. No freezing the liquid.
Room temp under 25°C: 7 days.
5 mg vial = 10 shots at 2 mg.
Label the date. Toss if cloudy or particulate.
Adjusting Doses by Bodyweight
80 kg is baseline.
- Under 70 kg: Drop 20% (2 mg to 1.6 mg)
- 90+ kg: Up 10-20% (2 mg to 2.4 mg)
Or 50 to 100 mcg/kg weekly loading.
90 kg: 4.5 to 9 mg/week split.
Monitoring During Cycle
Days 3-5: Fatigue or warm spots. Normal.
Week 2 on: Better flex if tendons are the target.
Bloodwork? CRP for inflammation check.
Headaches linger? Stop.
TB-500 with Injury-Specific Protocols
Tendons: 5 mg/week 6 weeks, then 2 mg/week 6.
Muscle: 7.5 mg/week load 4 weeks, BPC 400 mcg twice daily.
Recovery: 2.5 mg twice weekly, low and steady.
Common Reconstitution Mistakes
Shaking foams it up, ruins the peptide. Roll.
Vinegar or excess water? Weak brew.
Warm storage? Gone fast.
Over 1 ml? 1 ml syringe.
Advanced Frequency Tweaks
2-3 day half-life. Every 3 days flattens levels better than every other.
2 mg: Mon, Thu, Sun.
TB-500 calculator graphs it.
TB-500 Cycle Lengths
| Goal | Load Weeks | Maintenance Weeks | Total Cycle |
|---|---|---|---|
| Acute Injury | 4 | 4 | 8 |
| Chronic Tendon | 6 | 8 | 14 |
| Performance | 4 | 12 | 16 |
| Wellness | 2 | Ongoing low | Flexible |
Off 4-8 weeks between.
Sourcing and Vial Sizes
5 mg standard. 2 mg? Rare, skip.
Purity test if you can. Reputable sources matter.
Comparing to Other Peptides
TB systemic, BPC local. Stack 'em.
Sermorelin daily GH. TB twice weekly.
Nothing like PT-141 or DSIP. Straight repair.
AOD-9604 calculator for fat add-on.
Troubleshooting Low Response
Recheck math.
Week 3 no feel? 3 mg twice weekly.
SubQ, not IM—fat layer matters.
Stalled? MOTS-c for mitochondria.
From vial to shot, that's TB-500. Use the TB-500 calculator first.
Disclaimer: This information draws from research literature and is for educational purposes. Consult a healthcare professional before using any peptides. We are not medical advisors.
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