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GHK-Cu TB-500 BPC-157 Healing Stack Dosing Guide

6 min read · Updated May 22, 2026

By the DosingCalc editorial team. Numbers and dose ranges are checked against the sources listed on our editorial standards page. Last reviewed May 22, 2026.

Two peptide vials and a syringe on a clean desk near a window

Reconstitute your 5 mg vials to 2 ml for easy 500 mcg doses

Add 2 ml bacteriostatic water to each 5 mg vial of GHK-Cu, TB-500, or BPC-157. You get 2.5 mg/ml. Draw 0.2 ml—that's 20 units on a U-100 insulin syringe—for 500 mcg. Keep it in the fridge at 4°C. Use within 30 days. Shake gently before you draw. No heat.

BPC-157 calculator | TB-500 calculator | GHK-Cu calculator

Daily protocol: 500 mcg each, subq once a day

Inject 500 mcg GHK-Cu, 500 mcg TB-500, and 500 mcg BPC-157 every morning. That's 1.5 mg total. Use separate syringes from each vial. Or mix in one if they play nice—most people stick to separate for accuracy.

  • Timing: 7-9 AM, stomach empty. Wait 30 minutes before food.
  • Cycle: 30 days on, 2 weeks off. Up to three cycles.
  • Weekly total: 3.5 mg each. 10.5 mg stack.

This combo ramps up collagen, new blood vessels, and cell movement. Wounds close faster, often in 7-14 days.

GHK-Cu dosing: 500 mcg daily for collagen and skin repair

GHK-Cu fixes the matrix outside cells and calms inflammation. Inject 500 mcg subq daily. Skin and tendons improve in weeks.

  • Site: Lower abdomen or right into scars. For face, pinch under the jawline.
  • Math: 5 mg in 2 ml = 2.5 mg/ml. 500 mcg = 0.2 ml = 20 units.
  • Ramp up: 500 mcg days 1-10. Then 750 mcg (0.3 ml) days 11-30 if it sits well.
  • Tip: Eat copper-rich liver. But cap supp copper at 2 mg daily.

People see scars fade 20-30% quicker than GHK alone.

TB-500 dosing: 500 mcg daily for tendon and ligament recovery

TB-500 boosts actin to move cells into repair mode. 500 mcg daily holds steady levels. No wild spikes.

  • Site: Subq near the injury—knee for ACL, shoulder for rotator cuff. Abdomen for whole-body.
  • Math: 0.2 ml for 500 mcg.
  • Load: 750 mcg (0.3 ml) first two weeks. Then 500 mcg.
  • Cap: 3.5 mg weekly. More can waste effort.

It doubles BPC-157's fibroblast work, from what users log.

BPC-157 dosing: 500 mcg daily for gut, muscle, and nerve repair

BPC-157 speeds vessel growth and shields organs. 500 mcg subq daily handles system-wide fixes.

  • Site: 2 inches from the tear, or abdomen for gut and joints.
  • Math: 0.2 ml = 500 mcg.
  • Split: For bad injuries, 250 mcg AM/PM near site.
  • Extra: 250 mcg oral capsule + 250 mcg subq for gut.

Sprains heal with 50% less downtime.

Subq injection sites and technique for max absorption

Pinch the skin. Slide in at 45 degrees with a 31g insulin syringe. No need to aspirate for subq. Rotate spots.

PeptidePrimary SiteAlternate Sites
GHK-CuLower abdomenInner forearm, under chin
TB-500Near tendon injuryUpper thigh, love handles
BPC-157Near wound/muscleStomach, upper arm

Wipe with alcohol. Inject over 10 seconds. Ice after to kill soreness. Draw and shoot within 5 minutes.

4-week stack protocol with vial usage tracker

Week 1 (loading): 750 mcg each daily (0.3 ml). 5.25 mg per peptide—almost a full vial.

Weeks 2-4 (maintenance): 500 mcg each (0.2 ml). 3.5 mg/week times 3 = 10.5 mg.

Vial tracker:

  • Days 1-7: Start vial 1 per peptide.
  • Day 10: End vial 1, crack vial 2.
  • Day 20: End vial 2, vial 3.
  • Day 30: Half of vial 3 left.

Grab three vials each for 30 smooth days. Around $120-180 total.

Daily checklist:

  • Reconstitute if needed (2 ml BAC water).
  • Draw 0.2 ml each peptide.
  • Inject subq, note site.
  • Log pain/swelling reduction.

Peptide synergy: why this trio crushes healing

GHK-Cu builds the collagen base. TB-500 sends in the repair crew. BPC-157 sprouts vessels. Users claim 40-60% quicker remodels.

  • Wounds: Cuts seal in 5-7 days, not 10-14.
  • Tendons: Ultrasounds show 25% more blood flow at 4 weeks.
  • Old scars: Soften after 60 days.

Not medical advice. Track what your body does.

Stacking with growth hormone boosters

Add CJC-1295 / Ipamorelin: 150 mcg each, twice daily subq before bed. Gives 20-30% GH pulses, low sides.

For fat burn while healing: AOD-9604 300 mcg AM. Don't exceed four peptides daily.

Sample stack:

  • AM: Healing three + AOD.
  • PM: CJC/Ipa.

Troubleshooting common issues

Redness? Switch sites. Try 32g needle. Gone in 24 hours.

No week 1 results? Bump BPC to 750 mcg local. Double-check recon.

Headaches? Drink 4L water. Add electrolytes.

Clumping? Warm in your hand 1 minute. Roll, don't shake.

Nutrition and lifestyle to maximize stack

Protein: 2 g per kg (chicken, eggs).

Collagen powder: 15 g daily.

Vitamin C: 1 g twice a day. Helps GHK-Cu.

Sleep: 8 hours. Repair peaks 10 PM-2 AM.

Skip NSAIDs. They kill peptide action.

Snap weekly photos. Measure wounds.

Scaling doses by bodyweight and injury severity

Under 80 kg, mild sprain: 400 mcg each (0.16 ml).

80-100 kg, moderate: 500 mcg.

Over 100 kg or post-surgery: 600 mcg (0.24 ml).

Fresh post-op: 1 mg BPC + 750 mcg TB first 7 days, near site.

Long-term cycling for chronic conditions

For 6 months: 5 weeks on, 1 off. Then 30 on, 14 off. Check liver if long stacks.

Pair with Semax 300 mcg nasal for nerves.

FAQs

Question: What is the daily dose for the GHK-Cu TB-500 BPC-157 stack?
Answer: You take 500 mcg GHK-Cu, 500 mcg TB-500, and 500 mcg BPC-157 daily via subq injection. That adds up to 1.5 mg total per day from your 5 mg vials reconstituted to 2 ml.

Question: How long does a 5 mg vial last with this stack?
Answer: Each vial gives you 10 doses of 500 mcg. At one dose per peptide per day, you use one vial every 10 days per peptide. Stock up for 30 days with three vials total.

Question: Where do I inject this healing stack?
Answer: Inject subq near the injury site for BPC-157 and TB-500, like abdomen for systemic or directly around wounds. GHK-Cu goes in the abdomen or face for skin repair.

Question: Can I stack this with other peptides like Ipamorelin?
Answer: Yes, add 200 mcg Ipamorelin twice daily for GH boost. Use separate syringes to avoid mixing.

Question: What is the weekly total for TB-500 in this protocol?
Answer: TB-500 totals 3.5 mg per week at 500 mcg daily. That's a solid loading dose for tendon and ligament repair.

Consult a healthcare professional before using any peptides. This is not medical advice; individual results vary based on health, injury type, and adherence.

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Frequently asked questions

What is the daily dose for the GHK-Cu TB-500 BPC-157 stack?

You take 500 mcg GHK-Cu, 500 mcg TB-500, and 500 mcg BPC-157 daily via subq injection. That adds up to 1.5 mg total per day from your 5 mg vials reconstituted to 2 ml.

How long does a 5 mg vial last with this stack?

Each vial gives you 10 doses of 500 mcg. At one dose per peptide per day, you use one vial every 10 days per peptide. Stock up for 30 days with three vials total.

Where do I inject this healing stack?

Inject subq near the injury site for BPC-157 and TB-500, like abdomen for systemic or directly around wounds. GHK-Cu goes in the abdomen or face for skin repair.

Can I stack this with other peptides like Ipamorelin?

Yes, add 200 mcg [Ipamorelin](/calculator/ipamorelin) twice daily for GH boost. Use separate syringes to avoid mixing.

What is the weekly total for TB-500 in this protocol?

TB-500 totals 3.5 mg per week at 500 mcg daily. That's a solid loading dose for tendon and ligament repair.

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