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HGH Fragment 176-191 vs AOD-9604 Fat Loss Dosing

7 min read · Updated May 22, 2026

By the DosingCalc editorial team. Numbers and dose ranges are checked against the sources listed on our editorial standards page. Last reviewed May 22, 2026.

Photograph illustrating hgh fragment 176-191 vs aod-9604 fat loss dosing

HGH Fragment 176-191 and AOD-9604 cut straight to fat loss. They copy HGH's fat-burning part without the muscle gains or blood sugar mess. Reconstitute 5 mg vials the same. Dose in mcg ranges that overlap. Inject subQ every day. I like HGH Frag for stubborn belly fat—250 to 500 mcg hits hard. AOD-9604 keeps fat oxidation going steady at 300 mcg, split up. Grab our AOD-9604 calculator or check the protocols below. Dial in what fits you.

Reconstitute your 5 mg vials right

Take a 5 mg vial of HGH Fragment 176-191 or AOD-9604. Bacteriostatic water only. No plain sterile stuff.

  1. Wipe the vial top with an alcohol swab.
  2. Draw 2 ml bacteriostatic water into a 3 ml syringe.
  3. Drip the water slowly down the side of the vial. Don't blast the powder.
  4. Let it sit 10 minutes. Roll gently to mix. Skip the shaking.
  5. You get 5 mg in 2 ml. That's 2500 mcg per ml, or 250 mcg per 0.1 ml.

Use a 1 ml insulin syringe with 0.01 ml marks. 0.1 ml pulls 250 mcg. 0.2 ml gets 500 mcg. Stick mixed vials in the fridge at 2 to 8°C. They last 30 days. Freeze unopened ones at -20°C for 6 months. Let them warm to room temp before you draw.

For exact pulls, hit the AOD-9604 calculator. Or check the HGH calculator for stacks.

HGH Fragment 176-191 dosing protocols

Run HGH Frag at 250 to 500 mcg a day. Split doses over 300 mcg to keep levels even. Go by weight: 2 to 5 mcg per kg.

  • 70 kg person: 140 to 350 mcg daily. Start at 250 mcg.
  • 80 kg: 160 to 400 mcg. Try 300 mcg.
  • 90 kg: 180 to 450 mcg. Aim for 350 mcg.
  • 100 kg: 200 to 500 mcg. Don't go past 500 mcg.

Morning protocol

Shoot 250 to 300 mcg first thing. Empty stomach. Wait 20 minutes before eating. Do it 5 to 7 days a week. Cycle 4 to 8 weeks on, 2 weeks off. Fasted morning shots kick fat loose all day.

For an 80 kg person, days 1 to 28:

  • 6 AM: 0.12 ml (300 mcg) subQ into belly fat.
  • Black coffee's fine. No calories until 7 AM.

Pre-workout protocol

30 minutes before cardio or weights: 250 to 400 mcg. Peaks right when you need it for fat burn. 5 days on, 2 off.

75 kg gym rat example:

  • 5 PM workout: Inject 0.1 ml (250 mcg) at 5:30 PM.
  • Hit 45 minutes steady cardio at 65% max heart rate.

Stack it with the CJC-1295 / Ipamorelin calculator at 100 mcg each before bed. Pumps natural GH. No clash.

AOD-9604 dosing protocols

AOD-9604 runs 250 to 500 mcg daily. Split morning and afternoon most times. By weight: 3 to 6 mcg per kg.

  • 70 kg: 210 to 420 mcg. Start 300 mcg split.
  • 80 kg: 240 to 480 mcg. Total 300 mcg.
  • 90 kg: 270 to 540 mcg. Max 400 mcg split.
  • 100 kg: 300 to 600 mcg. Keep under 500 mcg.

Splits even out lipolysis.

Morning protocol

200 to 300 mcg when you wake up. Empty stomach. Optional second dose 4 hours later. 5 on, 2 off. Cycles last 4 to 12 weeks.

80 kg daily:

  • 7 AM: 0.12 ml (300 mcg) subQ.
  • No food for 30 minutes after.

Pre-workout protocol

150 mcg 30 minutes before training. Add 150 mcg morning. Targets fat during workouts.

70 kg pre-gym:

  • 6 AM: 0.06 ml (150 mcg).
  • 4 PM: 0.06 ml (150 mcg) before cardio.

AOD handles longer runs—up to 12 weeks. Tweak volumes with the AOD-9604 calculator.

Side-by-side comparison table

AspectHGH Fragment 176-191AOD-9604
Daily Dose250 to 500 mcg250 to 500 mcg split
mcg/kg2 to 53 to 6
Best TimingMorning fastedMorning + afternoon
Vial Reconstitution (5 mg / 2 ml)250 mcg / 0.1 ml250 mcg / 0.1 ml
Cycle Length4 to 8 weeks4 to 12 weeks
Fat TargetStubborn belly, visceralOverall oxidation
Injection Frequency1 to 2x day1 to 2x day

Inject subQ into low-fat spots—abdomen or thigh. Rotate sites.

Mcg per kg calculations explained

Match doses to your weight. No waste, better results.

Formula: Target mcg/kg times your kg equals daily total.

HGH Frag at 4 mcg/kg moderate:

  • 60 kg: 240 mcg (0.1 ml)
  • 85 kg: 340 mcg (0.14 ml)
  • 110 kg: 440 mcg (0.18 ml)

AOD-9604 at 4 mcg/kg, split twice:

  • 60 kg: 120 mcg x2 (0.05 ml each)
  • 85 kg: 170 mcg x2 (0.07 ml each)
  • 110 kg: 220 mcg x2 (0.09 ml each)

Under 60 kg? Start 2 mcg/kg. Over 110 kg? Cap total at 500 mcg. Returns drop off.

Stack with the Semaglutide calculator for appetite control next to these fat burners.

Morning injections: why and how

Morning hits mimic fasting GH pulses. Inject subQ 2 inches from your navel. Pinch skin. 45-degree angle. 29-31G needle.

80 kg protocol for either:

  1. Wake up. Pee. Skip food.
  2. Draw 0.12 ml (300 mcg).
  3. Inject. Hold 5 seconds.
  4. 20 minutes later: Water or coffee.

Run 6 days a week. Measure waist weekly. Look for 0.5 to 1 cm drop in 4 weeks with diet.

Morning HGH Frag plus a fasted walk? Lipolysis goes nuts. AOD morning keeps the burn steady.

Pre-workout protocols for max fat burn

Hit it 30 minutes before exercise. Cardio's best—LISS 30 to 45 minutes at 60 to 70% max HR.

HGH Frag pre-workout for 90 kg:

  • Draw 0.14 ml (350 mcg).
  • Inject thigh.
  • 30 minutes later: 40 minutes bike or incline walk.

AOD split pre-workout:

  • Morning 150 mcg.
  • Pre: 200 mcg.
  • Total 350 mcg.

Post-workout protein shake is fine. Skip carbs if you're cutting.

Add TB-500 calculator at 2.5 mg twice a week for recovery on heavy training.

Storage tips that save your vials

  • Unmixed 5 mg vials: Room temp short-term. Fridge 2 to 8°C best. Freezer -20°C up to 1 year.
  • Mixed: Fridge only at 2 to 8°C. Use in 28 days. No freezing after mixing.
  • Travel: Cooler bag with ice packs. Stay under 37°C.
  • Syringes: Keep dry. New one each time.
  • Light: Store vials in the dark. Brown bag works.

Bad storage clumps the powder or makes it dissolve slow. Toss it.

Stacking for better fat loss

Solo's solid. Stacks speed it up.

Track with weekly photos, scale, tape. No change in 2 weeks? Bump 10%.

Diet and training to amp results

Peptides pop in a 500 kcal deficit. 1.6 g protein per kg. 150 minutes cardio a week.

80 kg cutting day:

  • Fast till noon after 300 mcg morning.
  • Meal 1: 40 g protein shake.
  • Workout: 45 min cardio after pre dose.
  • Total calories: 2200.

App for macros. Sleep 7 to 9 hours for GH pulses.

Troubleshooting common issues

Redness? Ice 2 minutes after. Rotate sites. Water retention? Cut sodium—it's rare. No results? Add 50 mcg or check your mix. Headaches? Drink more. Drop dose.

Advanced protocols

12-week AOD lean-out for 85 kg: Weeks 1-4: 300 mcg AM. Weeks 5-8: 200 mcg AM + 150 mcg pre. Weeks 9-12: 250 mcg AM + 200 mcg pre.

HGH Frag burst: 500 mcg AM for 4 weeks. Off 2. Repeat.

Women: Cut doses in half—125 to 250 mcg to start.

Consult a healthcare professional before using any peptides. These protocols are for informational purposes based on user reports and not medical advice.

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Frequently asked questions

Which is better for fat loss: HGH Fragment 176-191 or AOD-9604?

Both target fat loss without HGH's growth effects. HGH Fragment 176-191 shines at 250 to 500 mcg daily for stubborn fat. AOD-9604 works well at 300 mcg per day split into doses. Pick based on your routine—morning for HGH Frag, pre-workout for AOD.

How do I reconstitute a 5 mg vial of these peptides?

Add 2 ml bacteriostatic water to a 5 mg vial for 250 mcg per 0.1 ml on both. Use a 1 ml insulin syringe. Store refrigerated after mixing.

What's the mcg per kg dose for HGH Fragment 176-191?

For an 80 kg person, 3 mcg per kg means 240 mcg daily. Round to 250 mcg. Scale up or down from there.

Can I stack these with other peptides like Ipamorelin?

Yes, stack HGH Frag or AOD with [Ipamorelin calculator](/calculator/ipamorelin) at 200 mcg nightly for GH boost without interfering with fat loss.

Morning or pre-workout—which timing works best?

Morning injections on an empty stomach mimic fasting fat burn for both. Pre-workout 30 min before cardio amps lipolysis during exercise.

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