A 5 mg vial of sermorelin with 2 ml bacteriostatic water means 250 mcg per 0.1 ml on your insulin syringe. Ipamorelin and CJC-1295 no DAC work the same way. Stack at 100 mcg each nightly: pull 0.1 ml from each vial. Total 0.3 ml in one shot before bed.
These peptides make sense together. Sermorelin wakes up your pituitary. Ipamorelin ramps GH without jacking cortisol. CJC-1295 no DAC stretches that pulse longer. You get 2-3 times the natural GH bump compared to running one alone. I like it because it's your own GH, not some external flood.
Peptide Reconstitution: Step-by-Step for 5 mg Vials
Use 2 ml bacteriostatic water per 5 mg vial. That gives you 250 mcg per 0.1 ml. Dead simple on a 1 ml insulin syringe with 100 unit marks.
- Pop the tops off sermorelin, ipamorelin, and CJC-1295 vials.
- Draw 2 ml water into a 3 ml syringe.
- Needle in at 45 degrees. Drip the water down the glass side. Don't blast it straight in, or it foams.
- Let it sit 5 minutes. Swirl gently. Dissolves in under 10. Fridge at 2-8 C.
You end up with 5 mg / 2 ml = 2.5 mg/ml = 250 mcg per 0.1 ml.
- 100 mcg = 0.04 ml (4 units)
- 200 mcg = 0.08 ml (8 units)
- 300 mcg = 0.12 ml (12 units)
Label each vial with the date and "250 mcg/0.1 ml." They last 28 days in the fridge. Hit the Sermorelin calculator, Ipamorelin calculator, or CJC-1295 / Ipamorelin calculator to confirm.
Beginner Stack: Sermorelin + Ipamorelin Nightly Protocol
New to this? Start simple. It matches your body's own GH rhythm. No overload.
Daily Dose:
- Sermorelin: 100 mcg (0.04 ml)
- Ipamorelin: 100 mcg (0.04 ml)
Syringe Pull: 0.04 ml sermorelin, then 0.04 ml ipamorelin into one 1 ml insulin syringe. Total 0.08 ml. SubQ in the abdomen, 2 inches from your navel. Rotate spots.
Timing: 30 minutes before bed. Empty stomach for 2 hours. No food. Hit bed by 11 PM to catch the GH wave.
Weekly Schedule:
- Monday to Friday: Full dose.
- Saturday/Sunday: Half dose (50 mcg each) or skip.
Cycle Length: 8 weeks on, 4 weeks off. Sleep improves first. Skin and recovery follow in 2-4 weeks. Bump to 200 mcg each after week 4 if it feels good.
One 5 mg vial at 100 mcg/night lasts 50 nights. Grab two each to start.
Intermediate Stack: Add CJC-1295 for Pulse Extension
Mastered the beginner? Add CJC-1295 no DAC. It drags GH release out to 3-4 hours instead of 1.
Daily Dose:
- Sermorelin: 100 mcg (0.04 ml)
- Ipamorelin: 100 mcg (0.04 ml)
- CJC-1295 no DAC: 100 mcg (0.04 ml)
Syringe Pull: All three in one syringe. 0.12 ml total (12 units). Pinch skin. 45-degree angle. Push slow.
Timing: Same spot before bed. Skip carbs after. Water or black coffee is fine.
Ramp-Up:
Week 1-2: 100 mcg sermorelin + 100 mcg ipamorelin + 50 mcg CJC (0.02 ml).
Week 3+: 100 mcg each.
Cycle: 12 weeks on, 4-6 weeks off. Watch your waist, energy, sleep.
This combo nails those GH peaks. With decent diet, folks drop 10-20% body fat in 3 months.
Advanced Stack: Higher Doses for Max GH Release
Ready to push? Go 200-300 mcg each. 600-900 mcg total nightly. Potent. Sustainable if you ease in.
Daily Dose:
- Sermorelin: 200 mcg (0.08 ml)
- Ipamorelin: 200 mcg (0.08 ml)
- CJC-1295 no DAC: 200 mcg (0.08 ml)
Syringe: 0.24 ml total (24 units). If your belly balks, split: 0.12 ml abdomen, 0.12 ml thigh.
Timing: Half at 8 PM, half at 10 PM. Or one shot 30 min before bed.
Monitoring: Fast water-only 3 hours before. Get weekly IGF-1 bloods if you can. Aim 200-300 ng/ml.
Cycle: 10 weeks at 200 mcg. Drop to 100 mcg weeks 11-12. 4 weeks off. Each vial lasts 25 nights.
Lift 4x a week. You get killer sleep and muscle repair. Tweak with the Ipamorelin calculator.
Nightly Injection Protocols: Make It Routine
Stick to it. Here's a 30-day plan.
Prep Kit:
- 1 ml insulin syringes (30g, 1/2 inch)
- Alcohol swabs
- 3 ml syringe for water
- Sharps container
Evening Routine:
7 PM: Last meal. Protein, fats. Low carb.
9 PM: Swab. Draw sermorelin first (it's clearest), ipamorelin, CJC.
9:30 PM: Inject. Lights out by 10.
Site Rotation:
- Night 1-3: Lower left abdomen
- 4-6: Lower right abdomen
- 7-9: Upper left thigh
- Repeat
No redness means you're good. Mild itch fades after week one. Ice 30 seconds after if it stings.
Travel Hack: Pre-fill day-of only. Stability drops otherwise. Double-bag in a cooler.
Nail dosing with the CJC-1295 / Ipamorelin calculator.
Syringe Calculations for Common Doses
For 250 mcg/0.1 ml. Burn these in.
| Dose per Peptide | Volume per Vial | Total for Stack (3 Peptides) | Syringe Mark (Units) |
|---|---|---|---|
| 100 mcg | 0.04 ml | 0.12 ml | 12 units |
| 150 mcg | 0.06 ml | 0.18 ml | 18 units |
| 200 mcg | 0.08 ml | 0.24 ml | 24 units |
| 250 mcg | 0.1 ml | 0.3 ml | 30 units |
| 300 mcg | 0.12 ml | 0.36 ml | 36 units |
Over 0.3 ml? Split or dilute to 3 ml water (167 mcg/0.1 ml, recalc).
Clear solution? Good. Cloudy, toss it.
Customize at Sermorelin calculator.
Cycle Lengths and Breaks: Avoid Desensitization
Receptors tire after 12 heavy weeks. Here's the plan.
Beginner (100 mcg each): 8 weeks on / 4 off. Three cycles a year.
Intermediate (100-150 mcg): 10 weeks on / 4 off.
Advanced (200+ mcg): 8-10 weeks on / 6 off.
Break Boost: 4 weeks solo ipamorelin at 100 mcg, or total off. 7-day water fast resets if you want.
Cycle Off Signs: Recovery stalls. Sleep worsens. Glucose climbs. IGF-1 over 350 ng/ml, ease up.
Six months in, fat melts, muscle sticks. Switch to TB-500 calculator for injuries.
Troubleshooting Common Issues
No effects by week 2? Check reconstitution. Shake clumps harder. Bump sermorelin to 150 mcg.
Pain? Warm vial in hand 2 min. Try 31g needle.
Headaches? Drink 4 L water daily. Cut CJC to 50 mcg.
Crystallizing vial? 37 C water bath, swirl.
Better than straight HGH. Compare at HGH.
Stacking with Other Peptides
After 4 weeks:
- Recovery: 250 mcg BPC-157 AM (BPC-157 calculator).
- Fat Loss: 300 mcg AOD-9604 post-workout (AOD-9604).
- Sleep: 100 mcg DSIP with stack (DSIP).
Skip GHRP-6/2, kills ipamorelin vibe. Semaglutide fine for fat loss (Semaglutide calculator).
Expected Results Timeline
- Week 1: Sleep deepens. Dreams pop.
- Week 3: Workouts recover fast. Skin looks alive.
- Week 6: 1-2% fat down. Strength jumps 5-10%.
- Week 12: IGF-1 up 50-100% from baseline.
1 g protein per lb bodyweight. 20% calorie cut. Track it.
FAQs
Question: How much bacteriostatic water for a 5 mg CJC vial?
Answer: Exactly 2 ml. Yields 250 mcg per 0.1 ml—perfect for 100 mcg at 4 units.
Question: Can women use this sermorelin stacking protocol?
Answer: Yes, start 50-75 mcg each. Same timing. Cycle 6-8 weeks.
Question: SubQ vs IM for GH peptide dosing?
Answer: SubQ abdomen best—steady absorption. IM faster but unnecessary.
Question: Store mixed stack in syringe?
Answer: No. Draw fresh nightly. Fridge vial only.
Consult a healthcare professional before starting any peptide regimen. These protocols are for informational purposes based on common user practices. Individual results vary.