Sermorelin Ipamorelin CJC-1295 Stacking Dosing Protocols

A 5 mg vial of sermorelin reconstituted with 2 ml bacteriostatic water gives you 250 mcg per 0.1 ml on your insulin syringe. Ipamorelin and CJC-1295 no DAC reconstitute the same way. To stack at 100 mcg each nightly, pull 0.04 ml (4 units) from each vial for 0.12 ml total, and inject it in one shot before bed.

The reason these three pair well comes down to how they work. Sermorelin wakes up your pituitary (Walker 2006, Clin Interv Aging, PMID 18046908), while ipamorelin raises GH without pushing cortisol up with it (Raun KK et al. 1998, Eur J Endocrinol, PMID 9849822). CJC-1295 no DAC keeps the pulse short. Pairing a GHRH analog with a GHRP or ghrelin agonist releases more GH than either does on its own, somewhere around 2-3 times the bump in preclinical work, though the reported numbers vary a lot by agent and dose (Raun KK et al. 1998, Eur J Endocrinol, PMID 9849822). What I like about the approach is that it's your own GH rather than an external flood.

Peptide Reconstitution: Step-by-Step for 5 mg Vials

Use 2 ml bacteriostatic water per 5 mg vial. That gives you 250 mcg per 0.1 ml, which is easy to read off a 1 ml insulin syringe with 100 unit marks.

1. Pop the tops off sermorelin, ipamorelin, and CJC-1295 vials.
2. Draw 2 ml water into a 3 ml syringe.
3. Put the needle in at 45 degrees and let the water run down the glass side. If you blast it straight in, it foams.
4. Let it sit 5 minutes, then swirl gently. It dissolves in under 10. Store it in the fridge at 2-8 C.

You end up with 5 mg / 2 ml = 2.5 mg/ml = 250 mcg per 0.1 ml.

• 100 mcg = 0.04 ml (4 units)
• 200 mcg = 0.08 ml (8 units)
• 300 mcg = 0.12 ml (12 units)

Label each vial with the date and "250 mcg/0.1 ml." The usual rule of thumb is about 28 days refrigerated, which comes from Bacteriostatic Water for Injection USP labeling and USP <797> beyond-use conventions rather than peptide-specific clinical data. Check your marks with the Sermorelin calculator, Ipamorelin calculator, or CJC-1295 / Ipamorelin calculator.

Beginner Stack: Sermorelin + Ipamorelin Nightly Protocol

If you're new to this, start simple. This pairing tracks your body's own GH rhythm and won't overload anything.

Daily Dose:

• Sermorelin: 100 mcg (0.04 ml)
• Ipamorelin: 100 mcg (0.04 ml)

Syringe Pull: 0.04 ml sermorelin, then 0.04 ml ipamorelin, into one 1 ml insulin syringe for a total of 0.08 ml. Inject subQ in the abdomen, 2 inches from your navel, and rotate spots between injections.

Timing: 30 minutes before bed, on an empty stomach with nothing eaten for the prior 2 hours. Get to bed by 11 PM to catch the GH wave.

Weekly Schedule:

• Monday to Friday: Full dose.
• Saturday/Sunday: Half dose (50 mcg each) or skip.

Cycle Length: 8 weeks on, 4 weeks off. Sleep tends to improve first, with skin and recovery following over the next 2-4 weeks. If it's going well, you can bump to 200 mcg each after week 4.

One 5 mg vial at 100 mcg a night lasts 50 nights, so pick up two of each to start.

Intermediate Stack: Add CJC-1295 for Pulse Extension

Once the beginner stack feels routine, add CJC-1295 no DAC (Mod GRF 1-29). This is the short-acting variant, with a half-life around 30 min, and you add it to keep the GH release brief and pulsatile rather than to stretch out a single pulse. The sustained 3-4 hour and multi-day elevation comes from CJC-1295 WITH DAC (t½ ~6-8 days), which is a different molecule (Teichman SL et al. 2006, J Clin Endocrinol Metab, PMID 16352683).

Daily Dose:

• Sermorelin: 100 mcg (0.04 ml)
• Ipamorelin: 100 mcg (0.04 ml)
• CJC-1295 no DAC: 100 mcg (0.04 ml)

Syringe Pull: Draw all three into one syringe for 0.12 ml total (12 units). Pinch the skin, go in at a 45-degree angle, and push slowly.

Timing: Same spot before bed. Skip carbs afterward; water or black coffee is fine.

Ramp-Up:
For weeks 1-2, run 100 mcg sermorelin + 100 mcg ipamorelin + 50 mcg CJC (0.02 ml), then move to 100 mcg each from week 3 on.

Cycle: 12 weeks on, 4-6 weeks off. Keep an eye on your waist, energy, and sleep.

This combo sharpens the GH peaks. Some users anecdotally report gradual fat loss over a cycle with a decent diet, but there's no controlled trial data in healthy adults, so treat figures like "10-20% body fat in 3 months" as community-reported rather than established.

Advanced Stack: Higher Doses for Max GH Release

If you want to push harder, run 200-300 mcg each, which is 600-900 mcg total a night. It's strong, but manageable as long as you ease into it.

Daily Dose:

• Sermorelin: 200 mcg (0.08 ml)
• Ipamorelin: 200 mcg (0.08 ml)
• CJC-1295 no DAC: 200 mcg (0.08 ml)

Syringe: 0.24 ml total (24 units). If that's too much for your belly to take in one spot, split it: 0.12 ml in the abdomen and 0.12 ml in the thigh.

Timing: Half at 8 PM and half at 10 PM, or a single shot 30 min before bed.

Monitoring: Stick to water only for the 3 hours before. Get weekly IGF-1 bloods if you can. The often-quoted target of 200-300 ng/ml isn't a validated goal for GH-secretagogue therapy; aim instead to stay within the age-adjusted reference range for your specific lab and assay.

Cycle: 10 weeks at 200 mcg, then drop to 100 mcg for weeks 11-12, followed by 4 weeks off. Each vial lasts 25 nights.

Lift 4x a week. Sleep and muscle repair tend to be the biggest payoffs. Tweak with the Ipamorelin calculator.

Nightly Injection Protocols: Make It Routine

The trick is consistency. Here's a 30-day plan to lean on.

Prep Kit:

• 1 ml insulin syringes (30g, 1/2 inch)
• Alcohol swabs
• 3 ml syringe for water
• Sharps container

Evening Routine:
7 PM: Last meal, mostly protein and fats, kept low carb.
9 PM: Swab, then draw sermorelin first (it's the clearest), followed by ipamorelin and CJC.
9:30 PM: Inject, and lights out by 10.

Site Rotation:

• Night 1-3: Lower left abdomen
• 4-6: Lower right abdomen
• 7-9: Upper left thigh
• Repeat

If there's no redness, you're fine. A mild itch usually fades after the first week, and you can ice the spot for 30 seconds if it stings.

Travel Hack: Only pre-fill on the day you're injecting, since stability drops if you fill ahead. Double-bag it in a cooler.

Nail dosing with the CJC-1295 / Ipamorelin calculator.

Syringe Calculations for Common Doses

These are for 250 mcg/0.1 ml. Worth memorizing.

If you're over 0.3 ml, split the shot or dilute to 3 ml water (167 mcg/0.1 ml, then recalculate).

A clear solution is fine to use. If it's cloudy, toss it.

Customize at Sermorelin calculator.

Cycle Lengths and Breaks: Avoid Desensitization

Receptors get sluggish after about 12 heavy weeks, so here's how to schedule around it.

Beginner (100 mcg each): 8 weeks on / 4 off. Three cycles a year.
Intermediate (100-150 mcg): 10 weeks on / 4 off.
Advanced (200+ mcg): 8-10 weeks on / 6 off.

Break Boost: Run 4 weeks of solo ipamorelin at 100 mcg, or take the time fully off. A 7-day water fast resets things too, if that's your thing.

Cycle Off Signs: Recovery stalls, sleep gets worse, and glucose climbs. As a practical heuristic rather than a primary-sourced limit, if IGF-1 climbs above your lab's age-adjusted reference range, ease up. The fixed 350 ng/ml cutoff you'll see quoted is community lore, and the ranges are age- and assay-dependent anyway.

Six months in, the typical pattern is fat coming off while muscle stays. If you pick up an injury, switch to the TB-500 calculator.

Troubleshooting Common Issues

No effects by week 2? Recheck your reconstitution, break up any clumps, and bump sermorelin to 150 mcg.
Pain? Warm the vial in your hand for 2 min and try a 31g needle.
Headaches? Drink 4 L water daily and cut CJC to 50 mcg.
Crystallizing vial? Give it a 37 C water bath and swirl.

A lot of people prefer this to straight HGH. You can compare at HGH.

Stacking with Other Peptides

After 4 weeks:

• Recovery: 250 mcg BPC-157 AM (BPC-157 calculator).
• Fat Loss: 300 mcg AOD-9604 post-workout (AOD-9604).
• Sleep: 100 mcg DSIP with stack (DSIP).

Skip GHRP-6 and GHRP-2 here, because they work against what ipamorelin is doing. They're less selective and raise ACTH and cortisol (and GHRP-6 drives appetite), which ipamorelin doesn't (Raun KK et al. 1998, Eur J Endocrinol, PMID 9849822). Semaglutide is fine alongside this for fat loss (Semaglutide calculator).

Expected Results Timeline

• Week 1: Sleep deepens and dreams get more vivid.
• Week 3: Workouts recover faster and skin looks better.
• Week 6: Anecdotal, community-reported gains. Some users note modest fat loss and small strength jumps (figures like "1-2% fat down, strength up 5-10%" are not from controlled trials).
• Week 12: A measurable IGF-1 rise is plausible, but response varies. Sermorelin produces a measurable IGF-1 rise that scales with dose, though there's no well-controlled sermorelin trial putting a precise percentage on it. The larger +50-100% figures you'll often see quoted come from more potent analogs like tesamorelin, not sermorelin; in a controlled trial, tesamorelin raised IGF-1 by 117% while staying in the physiological range (Baker LD et al. 2012, Arch Neurol, PMID 22869065).

Eat 1 g protein per lb of bodyweight on a 20% calorie cut, and track it.

FAQs

Question: How much bacteriostatic water for a 5 mg CJC vial?
Answer: Exactly 2 ml. That yields 250 mcg per 0.1 ml, which lands 100 mcg at 4 units.

Question: Can women use this sermorelin stacking protocol?
Answer: Yes. Start at 50-75 mcg each, keep the same timing, and run 6-8 week cycles.

Question: SubQ vs IM for GH peptide dosing?
Answer: SubQ in the abdomen is best for steady absorption. IM is faster but unnecessary here.

Question: Store mixed stack in syringe?
Answer: No. Draw it fresh each night and keep only the vial in the fridge.

Consult a healthcare professional before starting any peptide regimen. These protocols are for informational purposes based on common user practices. Individual results vary.

References

1. Raun KK, Hansen BS, Johansen NL, et al. "Ipamorelin, the first selective growth hormone secretagogue." Eur J Endocrinol, 1998. PubMed
2. Walker RF. "Sermorelin: a better approach to management of adult-onset growth hormone insufficiency?" Clin Interv Aging, 2006. PubMed
3. Teichman SL, Neale A, Lawrence B, et al. "Prolonged stimulation of growth hormone (GH) and insulin-like growth factor I secretion by CJC-1295, a long-acting analog of GH-releasing hormone, in healthy adults." J Clin Endocrinol Metab, 2006. PubMed
4. Baker LD, Barsness SM, Borson S, et al. "Effects of growth hormone-releasing hormone on cognitive function in adults with mild cognitive impairment and healthy older adults: results of a controlled trial." Arch Neurol, 2012. PubMed